Molecular Identification and Characterization of Antimicrobial Active Actinomycetes Strains from Some Egyptian Soils

Over classification problem of genus Streptomyces led the scientists to carry out many attempts to eliminate such problems. The current study aims at the molecular identification of antimicrobial activity of Actinomycetes from Egyptian soil. Among the 75 Actinomycetes collected isolates, 35 isolates by morphological and physiological studies were belonging to genus Streptomyces which were 64.3% exhibited activity against Gram positive bacteria, 48.5% showed activity towards Gram negative bacteria, 38.8% exhibited both Gram positive and negative bacteria and 80.85% isolates revealed significant antifungal activity from the total Streptomyces isolates. Analysis of the cell wall hydrolysates of the most potent two isolates showed the presence of LL-diaminopimelic acid and glycine. Molecular characterization of the two potent isolates was carried out based on PCR and sequencing. Sequence analysis indicated that two isolates shared 99-100% sequence identity to the 16S rRNA gene sequences with other Streptomyces taxons. Phylogenetic analysis revealed that the two isolates were belonging to genus Streptomyces within the same cluster. The obtained sequences were submitted to NCBI GenBank with accession numbers KM507575 and KM507576.